maria kaluzna

UJ MSc environmental protection, 

Few years of lab experience Nuscana Biotechnics Poznan -food, fodder viruses, allergens testing, WIOS Voivodeship Environmental Inspectorate Warsaw-laboratory testing water, Enfer Group Naas BVD TSE veterinary, IGSL soil tesing for engineers, Megazyme Neoger food carbohydrates, enzymes testing

As above

MARIA KALUZNA

Spire Hostel, 90-93 Marlborough Street Dublin D1, D01 X2W6 (possible relocation)

PROFESSIONAL SUMMARY

For over 3 years, I have become knowledgeable in overall laboratory function and maintenance in both food and biotechnology/pharmaceutical companies. Experience with cell-based bioassays, various ELISAs, qPCR, deviation, CAPA, out of specification (OOS). Revise and write Standard Operating Procedure (SOP). Well organized and detailed in data documentation. Experience in working in cGMP /GLP environments.

SKILLS

Write and Investigate deviation, CAPA and Out of Specification (OOS).
Critical reagents qualification.
Assay Troubleshooting.
LIMS Computer System
Aseptic Technique.
Good Laboratory Practices (GLPs).
Current Good Manufacturing Practices (cGMPs).
Good Documentation Practices (GDPs)
Assay Data Review.
Knowledge of ICH requirements
Read, Edit and Create Standard Operating Procedures (SOPs).
Method Transfer and Validation.
Accurate and Precise Pipetting technique.
Participate in the laboratory aspects of deviation investigations.
Problem solving to get to root cause of issues.
Provide support with audit/inspection requirements to ensure department compliance/readiness.
Quality Assurance · Quality Control · Analytical Biochemistry · Analytical Skills · Research
Proficient with software to include the QUMAS, Neogen-Mega-Calc™ software ChemWell®, Enfer Group Lims System, Nuscana-  Software Manual-Soxtherm Manager SX PC Gerhardt, Microsoft Office (Word, Excel, PowerPoint and Outlook).
Foreign languages: Native Polish, English, Intermediate Russian
Excellent Communication & Interpersonal skills

WORK HISTORY SUMMARY

BVD Laboratory technician

03 March 2023 – current (contract finishes on 03 June)

(NY) Bovine Viral Diarrhoea PCR positive in a dairy calf.
BVD is a viral disease which can infect cattle of any age. Infection of a pregnant cow or heifer can result in the birth of a persistently infected BVD positive calf.
Laboratory is independently accredited for BVD testing and the test methods used (ELISA or RT-PCR) will, in the great majority of cases, accurately detect the presence or absence of virus in tissue and blood samples when submitted and tested correctly.
PCR testing BVD samples
Pipetting using filter tips
LIMS
The BVD Antigen Capture ELISA (ACE) test is available for bovines only, and will determine if an animal is a PI or not. BVD ACE testing can be performed on an ear notch from cattle of any age, or on serum from a bovine 61 days old or older. The BVD PCR test is also available for cattle. It will detect both active infection and a PI; a positive result does not differentiate one from the other. 
Appropriate samples for BVD PCR include EDTA whole blood on cattle of any age, or serum or plasma from animals 61 days of age and older. If a cow is positive on a BVD PCR, a follow-up ACE test will be the next step to rule out a PI.
Camelid BVD screening is limited to the BVD PCR test, which is performed on EDTA whole blood on an animal of any age, or serum or plasma in llamas and alpacas 61 days of age and older.
BVD PCR testing is commonly used for cattle herd screening for BVD PI animals. Up to 10 cattle are pooled and tested at once. EDTA whole blood can be pooled from calves less than 61 days old, or serum, plasma or EDTA whole blood from cows over 61 days, can be pooled. 
Animals from the two age groups cannot be mixed, and sample types cannot be mixed. Please send individual samples to the lab. Samples are pooled upon arrival; we will not run pre-pooled samples. If the pool is positive, each sample will be run individually to identify the positive animal(s). At this time, there is no additional charge for the separate sample testing of a positive pool.
Pooling is the main element on the pipetting as we pool up to 24 samples into 1 pool – really precision pipetting
PCR Testing Method for detection of BVD in ear notches. This is recognised as being a highly sensitive way of detecting BVD in ear notch samples from young calves. PCR based test methods are unaffected by maternally derived antibodies from the mother’s colostrum.

QC Technician

Megazyme Neogen Europe Bray

28 Nov 2022-17 Feb 2023

QC testing
Calibration
Revise and review SOPs, test reports and protocols.
Core mission of developing and refining analytical methods used to measure the carbohydrates and enzymes in grain and cereal products that affect quality.
Working under the supervision of the Biochemistry Lead in Analytical Lab on specific assays testing, research challenges, including the development of new assay kits and enzyme products, calibration, lab maintenance.
Reviewed reagent preparation and buffers for all QC.
Reviewed equipment logs for QC laboratory.
Working in the biotechnology industry. Skilled in analytical method development, method validation and new product development, production and release. Strong project and team management skills with a particular focus in the field of Biochemistry/Enzymology.
Enzymes are proteins that catalyse biochemical reactions and are responsible for regulation of biological functions. Megazyme's chromogenic substrates provide the most convenient methods for the assay of hydrolytic enzymes, but polysaccharides and oligosaccharides can give the truest insight into their mechanism of action, active site requirement, binding affinities and activities.
Megazyme’s kits examples: 
K-AMIAR Ammonia Rapid, 
L-Arginine/Urea/Ammonia kit (K-LARGE) and NOPA kit (K-PANOPA).
K-FORM Formic Acid Assay Kit 
K-FRUGL D-Fructose/D-Glucose Assay Kit 
K-ACET Acetic Acid Assay Kit (ACS Manual Format)
L-Malic Acid Assay Kit (Manual Format)
D-Mannitol Assay Kit 
Food Cereals & Dietary Fiber, Malting, Brewing & Distilling Dairy & Infant Formula, Wine, Analytical Laboratories, Research, Biofuels, Animal Feed & Nutrition, Biotechnology & Enzyme Manufacturing, Analyte Enzyme Activity

Jul 2022-25 Nov “022

Bottom of Form

Laboratory Technician IGSL Ground Investigation & Geotechnical Specialists

Working within IGSL’s laboratory Division included laboratory testing works, maintenance / cleaning works, working in IGSL’s yard on sample management and other related duties 
Tests: Consolidation. Compaction, contamination, shear strength, determination of undrained shear strength, determination of shear strength laboratory vane method, permeability, shear box, grading, MCV moisture content value, liquid and limit plastic, plasticity index, liquid limit- cone penetrometer method, shrinkage limit tests TRL, samples preparations e.g. sieving, triaxial, determination of grains predominance undisturbed, bulk and disturbed small, density and particle density, chemical tests (pH, organic, carbonate, chloride miscellaneous contents/tests), oedometer, swelling pressure and settlement on saturation measurements in an oedometer apparatus, determination of swelling and collapse, labelling samples, Casagrande apparatus, ovens and heaters, , CBR etc.
Geotechnical industry -links with leading third level institutions (UCD, Carlow IT & University of Portsmouth). Rgeir laboratories in Naas perform testing and analysis of soils, rocks, aggregates, concrete and bituminous materials. 
INAB accreditation 
When you purchase land to build a home-the composition of your soil can affect everything from plumbing to foundations. There are soil tests to conduct as part of home construction process (soil composition, texture, structural stability
Soil mechanics is a branch of soil physics and implemented mechanics that describes the conduct of soils. It differs from fluid mechanics and solid mechanics inside the sense that soils include a heterogeneous aggregate of fluids (typically air and water) and particles (normally clay, silt, sand, and gravel) but soil might also incorporate natural solids and other rely. 
Shear box test is a testing method used to determine the shear strength of a soil sample. Since granular soils cannot be retrieved undisturbed, the soil is re-compacted inside a shear box. The soil’s friction angle is then determined by direct shear and the tests are carried out under saturated conditions.
The Atterberg limits are a fundamental measure of the essential water contents of an excellent-grained soil: its shrinkage restricts, plastic limit, and liquid limit. Relying on its water content material, soil can also appear in one in every of 4 states: strong, semi-stable, plastic and liquid. Atterberg limits may be used to distinguish among silt and clay and to differentiate among one-of-a-kind kinds of silts and clays. 
The shrinkage limit (SL) 
The plastic limit (PL) 
Plasticity index
Triaxial (shear) tests are an excellent way of measuring the mechanical properties of soil, rock and granular materials, with results used to solve a wide range of geotechnical engineering problems.
A triaxial test is a type of shear test that measures the mechanical properties of deformable solids, including cylindrical core soil, rock or granular material. The aim of a triaxial shear test is to establish the shear strength of a sample by replicating the in-situ stresses it would experience in the ground where it was taken from. In geotechnical engineering, triaxial tests provide design engineers with the data they need to calculate the effects of certain loads on their projects. By determining the shear strength of a soil or rock sample, we can work out how the ground beneath a foundation or embankment will respond to different loads – and the conditions under which it will experience shear failure.
For this reason, triaxial shear tests have a wide range of applications in the design of:
Consolidated Undrained triaxial test (CU)
Consolidated Drained triaxial test (CD)
Unconsolidated Undrained triaxial test (UU)
An oedometer check is a sort of geotechnical investigation executed in geotechnical engineering that measures a soil's consolidation residences. Oedometer tests are accomplished by way of making use of exceptional masses to a soil sample and measuring the deformation reaction. The consequences from these exams are used to expect how a soil inside the subject will deform in response to an alternate in powerful pressure.
Oedometer exams are designed to simulate the one-dimensional deformation and drainage conditions that soils experience inside the field. The soil pattern in an oedometer test is generally a round disc of diameter-to-peak ratio of about three:1. The pattern is held in a rigid confining ring, which prevents lateral displacement of the soil pattern, but permits the pattern to swell or compress vertically in response to modifications in applied load. 
The shear vane - is a method of measuring the undrained shear power of a cohesive soil. The take a look at is completed with equipment consisting of a rod with vanes set up to it this is inserted into the floor and turned around. A gauge on the top of the rod measures the torque required to reason failure of the soil and gives a conversion to shear strength. 
The shear vane includes a directly rod with four vanes on one end, organized in a cruciform sample, and a mixed handle/torque gauge. The rod is inserted into the soil to an intensity of 500mm and circled at a fee of between 6 and 12 levels in keeping with minute. Once the soil fails in shear the gauge shows the maximum torque implemented. The gauge is marked with a scale that converts the torque into a shear energy (measured in kiloNewtons in step with square metre). 
Soil classification,
Soil consolidation 
California bearing ratio, measure of the strength of the subgrade of a road or other paved area, and of the materials used in this construction, bearing load that penetrates a material to a specific depth compared with the load giving the same penetration into crushed stone, - using plunger 
Compressibility, permeability and durability test
Laboratory consolidation curve: logarithm of time fitting method
Laboratory consolidation curve: square root of time fitting method
Arrangement of apparatus for constant-head permeability test
Pinhole test apparatus
Consolidation test — specimen details Dispersibility — pinhole test
Hydrometer sedimentation
The constant-head permeability test 
Testing aggregates. 
Atterberg limits- The Atterberg limits can be used to distinguish between silt and clay and to distinguish between different types of silts and clays. The water content at which soil changes from one state to the other is known as consistency limits, or Atterberg's limit. These limits were created by Albert Atterberg,
Thermal conductivity/resistivity, 
Hydrometer,
Particle size analysis, sedimentation, . 
Gravity of soils weighing pycnometer

Laboratory TSE Technician Enfer Group Naas

10 Jan 2022-03 Jun 2022

The Enfer Group is an Irish technology group of companies which provide a range of products and services to the veterinary diagnostic industry.
Current R&D, production and quality systems operating from a 25,000 ft.² facility.
Laboratory testing service to the agriculture and food sectors in Ireland and internationally.
Operating to the ISO17025 quality standard, it is the largest private commercial veterinary laboratory in Ireland utilizing many unique systems and processes to deliver a high sample throughput with rapid results.
Enfer-TSE Technician j Transmissable Spongiform Encephalopathy (TSE) lab job covers: hands-on participation in animal diagnostic testing within a private testing facility, ELISA, processing samples for animal diagnostic testing, experience with RT-PCR data analytical tools, LIMS interaction, ISO accreditation standards and understanding of associated molecular biology laboratory techniques and technologies, preparing samples for loading onto machines, operating numerous instrumentations (Microplate Incubator, Microplate Washer, Microplate Absorbance Reader, Homogenizer, Aluminum Sealing Film, 92-Well Deepwell Microplate, Eppendorf Centrifuge, Heat Blocks, etc.)

• Testing for both Bovine Spongiform Encephalopathy and Scrapie Disease
• Maintenance of all customer and laboratory paperwork to ensure full sample traceability
• Grading of Bovine and Ovine samples according to guidelines
•Cutting samples in preparation for testing following by lysis and homogenisation.
•Preparation of reagents and washes for the assay including working plate diluent, conjugate, controls
• Use and maintenance of laboratory equipment
• Preparation and use of Internal Quality Controls
• Stock checks within the laboratory.
Transmissible spongiform encephalopathies (TSEs) are transmissible neurological diseases caused by tiny infectious agents called prions. Bovine spongiform encephalopathy (BSE) is a fatal disease of adult cattle that was first recognised in Great Britain in the 1980s. It is now rare in Europe. Scrapie is a naturally occurring infectious disease of sheep and goats. It has been recognised as a clinical disorder for more than 250 years.
The HerdChek BSE-Scrapie Ag Test is an antigen-capture enzyme immunoassay (EIA) for the detection of the abnormal conformer of the prion protein (PrPSc) in bovine, caprine and ovine postmortem tissues.

Obex, spleen and lymph node samples

Very simple sample preparation and EIA protocol—no proteinase K digestion, less equipment required, less hands-on time, results in 90 minutes

The IDEXX HerdChek* Bovine Spongiform Encephalopathy (BSE)-Scrapie Antigen Test Kit is an antigen-capture enzyme immunoassay (EIA) for detection of the abnormal conformer of the prion protein (PrPSc) in postmortem brain (obex preferred) tissues from bovines, small ruminants (sheep and goats) and cervids affected by BSE, scrapie, or chronic wasting disease (CWD). For small-ruminant applications, the test will also detect PrPSc in lymph node or spleen tissue; for cervids the test will also detect PrPSc in retropharyngeal lymph nodes (RPLN). It is designed to rapidly identify samples containing disease-associated PrPSc with minimal sample handling, and can be automated for high throughput applications. This kit is for veterinary use only.
Namely, PrPSc, being richer in beta sheet content, was found to be much more resistant to denaturation and proteolytic degradation than PrPC. Ever since, PrPSc has been detected either by immunohistochemistry after special pretreatments of tissue slices, which destroyed relevant PrPC epitopes, or by western blotting of brain homogenates after degradation of PrPC by proteinase K. Many other commercially available diagnostic immunoassays that have been developed still relay on PK digestion of PrPC. PrPSc-specific monoclonal antibodies have always represented an ideal approach for prion diagnostics development. Although the use of denaturation for PrPSc epitope revealing eliminates the need for PK digestion, it can be applied for more efficient elimination of PrPC and therefore for improved discrimination between TSE-positive and TSE-negative samples. PrPSc aggregates were only soluble when the concentration of denaturant was high enough. An important issue of immunoassaying brains is the fact that different parts of brain may vary greatly in the abundance of PrPSc, which was shown for animal and also for human brain. Some parts of infected brain may therefore contain only very low amounts of PrPSc. Because IHC is not a high-throughput method, protocols for detection of PrPSc from paraffin-embedded tissue by WB have been developed [35, 36]. They can readily be transferred to ELISA test format , enabling the analysis of larger number of samples compared to IHC. Sensitivity of an immunoassay depends not only on the sample preparation and treatment, but largely also on the detection system. As PrPSc is supposed to be present in extremely low amounts in these samples, immunoassays need to include an additional step of PrPSc enrichment [44-47]. In an effort to reach high sensitivity, the specificity of the method should not be neglected. Multiple repetitions of aggregation and sonication cycles enable multiplification of PrPSc to the level, detectable in WB. Two recent reports have shown the use of in vitro amplification of PrPSc in combination with immunoassays more sensitive than WB. Subsequently, newly formed PrPSc aggregates are captured by an aggregate-specific mAb in AS-ELISA in combination with FACTT where detection is performed via biotin-conjugated DNA template.
For small-ruminant applications, the test will also detect PrPSc in lymph node or spleen tissue; for cervids the test will also detect PrPSc in retropharyngeal lymph nodes.

Nature Protection – Volunteer

RDOS Regional Directorate of Environmental Protection in Poznań

Apr 2020 - May 2020

Nature 2000 sites. 
conducting matters related to the reconciliation of studies of spatial planning and directions, local zoning plans of municipalities, zoning plan of the province in order to prepare planning documents for protected areas.

Microbiologist/ Laboratory Technician

Nuscana Biotechnics Mrowino-Poznan internship

03 Dec 2019 – 03 Apr   2020

Tested food, cosmetics, medication, drugs, fodder, animal viruses, hormones, allergens, antibiotics
Conducted enzymatic tests, microcystin, nutritional value and fatty acids testing
Checked the total number of microorganisms, fungi and mould, salmonella, listeria, e. coli, coliforms etc.
In microbiology department we are checking total number of microorganisms, fungi and mould, Salmonella, Listeria, E. coli, Coliforms, Enterobacteriaceae, Enterococcus, Staphylococcus aureus, Bacillus cereus, Cronobacter, Vibrio, Shiga toxin and pathogens in water. It gives a lot of satisfaction to protect people health in terms of food ingredients, poisons, toxins, allergens, etc.
We test food, cosmetics, medication, drugs, fodder, animal viruses, hormones, allergens, antibiotics, inhibitory substances in milk, species of milk and meat, production hygiene, mycotoxins, GMO, food intolerances, enzymatic tests, microcystin, nutritional value and fatty acids etc.
ISO 17025:2017
Sample management
Calibration and checks reporting, authorization of reports
Test method development, modification, verification and validation
Statements of conformity or opinions and interpretations, environmental monitoring daily temperature readings, regular checks
Performed microbial analyses on domestic food aseptically to test for sterility, biocompatibility, toxicity, bioburden, sterility validation program and determined product shelf life.
Performed cell culture on agar plates, diagnosed antibodies using serology method, performed biochemical reactions on microorganism using API (Analytical Profile Index).
Sampled raw materials, finished goods, swabs from clients to test for mould, E. Coli, Staph, Salmonella, Listeria, Yeast, Fungi, Coli forms, Antibiotic and other microorganisms.
Used and maintained laboratory equipment like; ELISA tester, PCR, pH meter, conductivity meter, HPLC, separating funnel, incubator, centrifuge, colony counter, microscopes, autoclaves, controlled environmental incubator shaker and nitrogen extractor, Agilent bio analysers.
Water activity (aw)
NIR – Near-Infrared Analyzers Near-infrared (NIR) analyzers are most often used in the agri-food industry to test the quality of agricultural raw materials and finished products, i. processed food or feed. In this way, grain purchases, mills, feed producers or food production plants have the opportunity to adjust the price of raw materials to the quality and also gain the ability to control quality in production plants, e. during the production process. Cereal and flour analyzer: SpectraAlyzer GRAIN NEO The wavelength range (750-2400 nm) is ideally suited to the characteristics and matrices usually studied in the agri-food industry. Analyzers are used in various industries: grain buying, mills, feed mills, shot and oilcake plants, oil and fat producers, egg industry, pasta factories, bakery, meat processing plants, dairies and cheese factories, breweries, wine and alcoholic beverage producers, biogas plants and distilleries. • Autosampler for liquids for 180 samples The samples are tested by tansflection with a specific thickness of the light path. The sample is applied to the cuvette and covered with a microscope slide. •
SOFTWARE Application worx (AWX G2) The SpectraAlyzer is a powerful and versatile tool for NIR analysis that works well without any PC support. But in order to use NIR spectroscopy to its full potential it is necessary to tap into the additional processing power and storage capability of a PC. • Sample mineralization is the initial stage of many studies. In the process of mineralization, organic matter is removed and inorganic matter is stabilized in molecular form. Gerhardt equipment enables the mineralization of samples for further use for various purposes and for laboratories of various sizes. We can offer devices for manual mineralization, where the appropriate operations are performed manually, but also semi-automatic and automatic machines for medium and large number of samples; flasks can be distilled in the automatic VAPODEST distiller. • Most often, C. Gerhardt mineralizers are used for the determination of nitrogen or protein by the Kjeldahl method, but also for the determination of heavy metals.

Reagents, i. tablets with catalysts, antifoaming agents and consumables, i. mineralization flasks, heaters, glass elements. • Classic mineralizer. The set consists of 6-station heating plates with separate power regulators. Distillation can be carried out in the VAPODEST distillation machine, to which the flasks mentioned above are fitted. • A tap jet pump or TURBOSOG system should be used to evacuate fumes. • Classic mineralizer with distiller This set is suitable for simultaneous digestion followed by distillation. This 6-station set is equipped with flasks with a capacity of 500 and 750 ml set on the heating block. In addition, it has a bottle for decanting leftovers, a tap jet pump and a set of hoses.
TURBOTHERM mineralizer This device heats on the infrared principle and is a quick alternative to the KJELDAHLTHERM system. • TURBOTHERM is most often used for the mineralization of samples for the determination of nitrogen and protein by the Kjeldahl method at the micro and macro scale. The samples mineralized in the flasks can be directly distilled in an automatic VAPODEST distiller. • This equipment is also often used for the digestion of samples in inorganic acids and has a programmable panel to control the temperature and time of mineralization. • 
KJELDATHERM mineralizer • TURBOSOG Fumes formed during mineralization should be removed next to the place of their formation. The advantage of its use, in addition to avoiding dangerous gases, is the savings in tap water consumption, which is usually wasted by tap jet pumps.
In addition, TURBOSOG has the ability to adjust the suction force depending on the number of flasks used and the amount of organic matter in the flasks. • VACUSOG The main advantage of this new device is the fact that it does not use tap water at all to neutralize and separate dangerous mineralization fumes • Acid hydrolysis of fats (Weibull-Stoldt method) The protein-bound fat cannot be completely extracted with ether gasoline during Soxhlet extraction. To extract the total fat, the sample should be hydrolyzed by boiling it in hydrochloric acid. The total fat released in this way is drained and then the filters are extracted with ether gasoline.
Hydrotherm is the world's first and currently the only automatic device for the determination of total fat using the classic Weibull-Stoldt method. The whole process, from acid hydrolysis to filtration, takes place in a closed system, so this device can work outside the fume hood and laboratory personnel have no contact with hot reagents and acid vapors. Six samples can be hydrolysed simultaneously. 
DUMA – protein, nitrogen and carbon analyzers • Determination of nitrogen/protein content of whey DUMATHERM N Pro analyzes almost all sample types, both liquid and solid, and provides highly accurate results 
ELISA readers and scrubbers • The use of ELISA requires the use of a microplate scrubber and an optical density or fluorescence reader. • Stomachers-Depending on the sample weight or volume, adjust the size of the bags and the stomacher. • PCR Method for the detection of pathogenic organisms • DILUTORS Automatic dilution equipment • Microbiological tests Ø Compact Dry AQ For determining the number of heterotrophic bacteria in tap water and ultra-pure water. • Test swabs Compact Dry VP XSA Dry TC YMR YM • Bags for samples and Stomacher • PCR-based system designed for the detection of pathogenic organisms in food and environmental samples. 
FOOD INTOLERANCE TESTS - Food allergen detection tests>Alergo Test • Examine allergens using 4 different techniques, including the most important: ELISA. We also use PCR for some samples. • Alergo tests is the name of a group of tests used to perform rapid tests for the presence of food allergens in food, in swabs and in liquids after washing production lines. The tests contain all the necessary accessories and reagents. Specially selected extraction buffers open and stabilize allergenic proteins so that the ELISA reaction on the strip can take place as efficiently as possible.
Fiber + Vitamins and minerals.
Fat analysis in most matrices is preceded by the hydrolysis process.
Spectrum of fatty acids-test omega-3 and omega-6 fatty acids quantitatively using the accredited method.

Laboratory technician internship  

Voivodeship Inspectorate of Environmental Protection, WIOS in Warsaw, Poland

Aug 2004 – Dec 2004

Performing water, wastewater, sediments, soil and air samples analysis (e.g. in water -estimation of Cl-, PO43-, SO42-, NO3-, NO2-, Hg, phenols, hydrocarbons from crude)
Assisting voluntarily in Monitoring Division – entering data about air pollution and its sources onto database about the environment
Interaction with developers for creation of safer and cleaner for environment infrastructure techniques

The Provincial Inspectorate for Environmental Protection WIOŚ Warsaw Laboratory
Laboratory performs a full range of tests in the area of operation of the Provincial Inspectorate for Environmental Protection, resulting from threats and the need for control measurements of organizational units, needs resulting from extraordinary environmental threats and environmental monitoring. As part of the basic activities, tests of physico-chemical and biological indicators are carried out in samples:
surface water, groundwater and rainwater,
Wastewater
Soil
ambient air (immission),
waste and landfill gases (emissions),
dust (emission),
industrial waste and sewage sludge.

In addition, the WIOŚ Laboratory performs measurements of communication and industrial noise and electromagnetic radiation.

In order to guarantee impartial, independent research, the WIOŚ Laboratory carries out a comprehensive control of the obtained analysis results in accordance with the management system. Control of analytical work is carried out in accordance with the provisions of the Quality Book and the Book of General Procedures. Laboratories use reference standards, sampling control, quality control and analysis of samples of unknown concentration in routine work. The laboratories also participate in inter-laboratory external, domestic and foreign research.

Performing a wide range of analytical tests for different needs and in different samples allows the WIOŚ Laboratory to have modern control and measurement equipment.

The laboratory base includes the following (main) equipment:

gas chromatographs – detectors: FID, ECD and MS,
liquid chromatographs – UV, DAD and fluorescent detectors,
ion chromatographs (Dionex),
atomic absorption spectrometers: flame and flameless atomization (graphite cuvettes),
ICP spectrometers,
UV/VIS spectrophotometers,
analysers for total organic carbon, organic nitrogen and BOD5,
Automatic atmospheric air measuring stations - automatic analyzers: meteo conditions (temperature, wind direction and speed, humidity,
atmospheric pressure, insolation and rainfall), O3, CO, NOx, PM 2.5, PM 10, SO2 and BTX,
manual particulate matter collectors PM 2.5 and PM 10,
•analytical balances, conductivity meters, oxygen meters, pH meters, microscopes,
noise meters, electromagnetic field

Such modern equipment is operated in accordance with the intended purpose and quality control requirements. The WIOŚ Warsaw laboratory uses certified standards and reference materials, and the equipment is subjected to constant metrological control (legalization and calibration).

The above-mentioned equipment is provided with appropriate, constantly supervised premises and environmental conditions. Preparatory and analytical rooms have been separated, which makes it possible to perform research with the care assumed in the research methods and eliminates mutual adverse influence.

Tests and measurements are performed in accordance with Polish Standards, international standards and documented own test procedures. Regardless of the choice of the method of analysis, the principle applies that the tests are performed using reliable and validated methods. The validation of standards and test procedures involves, among other things, the determination and verification of the following parameters:

the limit of detection and quantification of the method,
repeatability and reproducibility of the method,
uncertainty of the method,
recovery testing,
quality control: duplicate samples, Shewhart cards, Cusum cards, R-cards and evaluation of random and systematic errors.

The basic principle of the work of the WIOŚ Warsaw Laboratory is to perform tests in accordance with the requirements of the Management System according to PN-EN ISO / IEC 17025: 2005 in order to achieve an appropriate level of reliability of the tests performed. All laboratories of the Provincial Inspectorate for Environmental Protection in Warsaw are accredited according to PN-EN ISO/IEC 17025:2005.                                    

EDUCATION SUMMARY

Jagiellonian University Institute of Chemistry

Master of Science environmental protection biology of environment (Hons)

01Oct 1997-31 Jul 2000            

MSc Thesis in Biotechnology in field of Plant Physiology and Development. Topic: ‘The inhibitors of the growth of Cyanobacteria Microcystis aeruginosa obtained from decomposition of dry mass of chosen plant species’, (grade 4.5, B+)

Bachelor of Science environmental protection chemistry of environment (Hons)

01 Oct- 25 Mar 2004

BSc Thesis in Chemistry of Environment in field of Work Safety.   Topic: ‘Luminosity and illumination, basic principles of work hygiene and physiology’, (grade 5, A)

Modules: General and Inorganic Chemistry, Zoology, Mathematics, English, Physics, Biology, Swimming, Athletics, Analytical Chemistry, IT, Botany, Ecology, Geology and Geochemistry, Meteorology and Climatology, Hydrology, Hydrobiology, Soil Science, Sustainable Development and Environmental Hazards, Microbiology, Physical Chemistry, Nature Conservation, Ecology of Agriculture, Environmental and Technologically Induced Radioactivity, Ecology of Population, Organic Chemistry, Toxicology, Work Safety, Biogeochemistry, Economic Principles of Environmental Protection, Environmental and Technologically Induced Radioactivity – Laboratory, Legal Foundations of Environmental Protection, Biological Monitoring, Chemical Technology and Engineering in Environmental Protection, Instrumental Analysis, Biological Wastewater Treatment, Biochemistry, Medical Aspects of Nature Conservation, Environmental Education, Seminar of Ecology, The Natural History of Bats, Ecological Basics of Hunting, Industrial Ecology, Catalysis and Catalysts in Environmental Protection, Physics of Energy Transformation Processes, Recultivation of Transformed Areas, Bromatology, Genetics, Evolutionary Biology, Toxicology of Environment, Nutritional Prevention Against Cancer, Oceanology, Ecology of Inland Water, Vibro-acustics Hazards, Industrial Impact on the Environment, Environmental Impact Assessment, Landscape Architecture and Space Planning, Agriculture Chemisation and Its Environmental Impact, Animals of Prey, Master Seminar, Management in National Parks, Plants Used by Man, Biology of Birds, Primatology, Polar Biology, Natural Philosophy, Master Laboratory.

For 3 years I was successful with securing a scientific scholarship which is granted only for top 20% of best students.

Research (besides MSc and BSc dissertations):

For my Seminar of Ecology I worked on the study case: ’Forest – wealth of the nation or citizen’. I was comparing the conditions of Polish private forests with Polish state forests. My research I was supporting with findings from other countries such as USA. We found out that Polish private forests were poor in terms of species and biodiversity, often neglected, at risk in term of health which was caused by their owners being not able to afford for better forest care.
For Polar Biology I researched on wasteful exploitation by humans on Arctic Sea, whaling and hunting on webbed.
When we were studying nature conservation, I have done some extra research on the role of national parks in nature protection and Olympic Games (Zakopane – Poland, 2006) as a problem for wildlife.

1999 Summer Internships:

Industrial Professional Placement in Public Utility Commission, Cracow, Poland

determination of metals in water. 
The Central Laboratory offers the following services:

physicochemical testing of water, 
microbiological testing of water, 
physicochemical tests of sewage, 
physicochemical and biological tests of sewage sludge and biologically active ones, 
sampling for testing, 
training and consultancy in the field of management system and technical competences in a research laboratory according to PN-EN ISO: 17025 and others resulting from requests for proposals.
The activity of the Laboratory is carried out on the basis of Polish Standards and Research Procedures. The laboratory is equipped with gas and ion chromatographs, analyser for the determination of organic carbon, atomic absorption spectrometer (AAS), UV-VIS spectrophotometers, pH meters, conductivity meters, water purification systems.

Natural Professional Placement in Wigierski National Park, Suwalki, Poland

Animal species protection, counting different species of animals

Learning Cloud

Advanced Certificate Zookeeping (online)

Jul 2021-current

Only 3.5 modules are left to complete the course

University of London Birkbeck College

Master of Science

Environmental Management

01 Oct 2011- Apr 2012

Attended first year of 2 years course part time studies – left due to having an accident in 2013

ACCOMPLISHMENTS

Collaborated with team in the development of projects.
For 3 years I was successful with securing a scientific scholarship which is granted only for top 20% of best students.

CERTIFICATIONS

Certificates in Health and Social Care, Autism, Dementia NVQ II links 2006-2012 
Certificate in Communication for conservation   2022 Team Conservation Careers                                                               Dr. Nick Askew and Lloyd Gofton
Legitimation of horse-riding instructor Poznan, Poland 1999

OTHER WORK

Support Worker Robina Health Care, Hindhead, Hampshire UK

Jun 2006-May 2007

As above

I am a graduate of the Jagiellonian University in Cracow in the field of environmental protection, Faculty of Chemistry, Faculty of Biology and Earth Sciences (Bachelor: 5, July 2000, Master: 4.5 March 2004). 

• Master's thesis - biotechnology, plant physiology, Cyanobacteria Microcystis aeruginosa cyanobacterial growth inhibitors obtained from the distribution of dry matter of straw and various plant species (grade 4.5, B +).

• Bachelor's thesis - environmental chemistry, health and safety at work, ergonomics. Light and lighting, basic principles of occupational hygiene and physiology (grade 5, A).

• Research in addition to bachelor's and master's theses):

o Ecology seminar - privatization of municipal forests in Poland - compared to forests in the USA. 'Forest - wealth of the nation or citizen'. We have proved in our results that private forests in Poland are poor in species and biodiversity, often abandoned, often found on the border of risk as 'unhealthy', sometimes due to financial shortages and inefficiencies in the cost of living

o Polar biology - hunting in the Arctic Sea region, whales, seals, pinnipeds

o National Parks - and the Olympiad (Zakopane - Poland, 2006, London, 2012).

Modules: General and Inorganic Chemistry, Zoology, Mathematics, English, Physics, Biology, Swimming, Athletics, Analytical Chemistry, IT, Botany, Ecology, Geology and Geochemistry, Meteorology and Climatology, Hydrology, Hydrobiology, Soil Science, Sustainable Development and Environmental Hazards, Microbiology, Physical Chemistry, Nature Conservation, Ecology of Agriculture, Environmental and Technologically Induced Radioactivity, Ecology of Population, Organic Chemistry, Toxicology, Work Safety, Biogeochemistry, Economic Principles of Environmental Protection, Environmental and Technologically Induced Radioactivity – Laboratory, Legal Foundations of Environmental Protection, Biological Monitoring, Chemical Technology and Engineering in Environmental Protection, Instrumental Analysis, Biological Wastewater Treatment, Biochemistry, Medical Aspects of Nature Conservation, Environmental Education, Seminar of Ecology, The Natural History of Bats, Ecological Basics of Hunting, Industrial Ecology, Catalysis and Catalysts in Environmental Protection, Physics of Energy Transformation Processes, Recultivation of Transformed Areas, Bromatology, Genetics, Evolutionary Biology, Toxicology of Environment, Nutritional Prevention Against Cancer, Oceanology, Ecology of Inland Water, Vibro-acustics Hazards, Industrial Impact on the Environment, Environmental Impact Assessment, Landscape Architecture and Space Planning, Agriculture Chemisation and Its Environmental Impact, Animals of Prey, Master Seminar, Management in National Parks, Plants Used by Man, Biology of Birds, Primatology, Polar Biology, Natural Philosophy, Master Laboratory.

For 3 years I was successful with securing a scientific scholarship which is granted only for top 20% of best students.