LOAI SIDDIG

Lecturer, King Fahd Medical Research Centre, King Abdul-Aziz University, Jeddah, KSA         

• Taught Molecular Biology and Microbiology to graduate-level Medical Laboratory Science students.
• Conducted assessments through quizzes, projects, and exams; provided one-on-one tutoring.
• Mentored students in lab-based molecular and Microbiology techniques to ensure practical proficiency

King Fahd Medical Research Centre, King Abdul-Aziz University, Jeddah, KSA

• Research and Technical Expertise: Conducted advanced molecular and microbiological techniques like PCR and ELISA for disease research and diagnostics, ensuring accuracy and adherence to protocols when handling infectious agents.
• Data Acquisition and Analysis: Utilized statistical software for data acquisition and quality control, documenting findings to support research with reliable, reproducible data.
• Lab Management and Quality Control: Managed lab inventory, quality control, and SOP updates to maintain diagnostic standards and align with current research methods.
• Leadership: Progressed from Research Assistant to Molecular Research Lab Supervisor, leading a team in molecular diagnostics, training junior staff, and promoting lab safety and continuous learning.

SKILLS

• Molecular Techniques: DNA & RNA (Extraction, Purification, & Concentration); PCR (Multiplex PCR, Multi Locus Sequencing Typing PCR, Real-Time PCR, Cloning PCR, Gradient PCR, ERIC PCR); Gel Extraction; Primer Design; Electrophoresis (Agarose Gel, Capillary Electrophoresis, Pulse Field Gel, and Polyacrylamide Gel); Sequencing; Molecular Cloning; Plasmid Isolation.
• Protein Techniques: Protein Purification; Protein Extraction; Western Blotting.
• Diagnostic Techniques: Bacterial DNA Culture, Identification, Isolation, and Antibiotic Susceptibility Test; ELISA; Molecular typing techniques (MLST, RFLP, PFGE); Vaccine Development.
• Laboratory Management: Preparation of Competent Cells; Lab inventory management; Quality control; SOP updates.

Medical Laboratory Technologist, Cardiothoracic Institute, National Cardiac Centre, Khartoum, Sudan 

• Served as a Laboratory Specialist in the Microbiology and Molecular Biology Department. Demonstrated exceptional proficiency and commitment in medical laboratory science.
• Contributed significantly to the diagnosis of COVID-19 and bacterial infections by conducting daily bench work in bacteriology, virology, and molecular testing.
• Ensured the precision and reliability of lab results, maintained and calibrated equipment, followed strict safety and quality control protocols, and mentored new laboratory staff and graduate students.

Medical Laboratory Technologist, Alshaab Teaching Hospital-Center for Cardiology and Respiratory Medicine, Sudan

• Receiving, labeling, and storing samples to be tested.
• Conducting diagnostic tests for COVID-19.
• Performing bacterial cultures and sensitivity testing.
• Ensuring the accuracy and reliability of laboratory results.
• Maintaining and calibrating laboratory equipment.
• Adhering to strict safety and quality control protocols.
• Collaborating with healthcare professionals to provide timely and accurate diagnostic information.

12/2021-01/2023

Medical Laboratory Technologist, Juba Military Hospital, Juba, South Sudan 

• Conducted diagnostic tests for various infectious diseases using blood, stool, and urine samples
• Primarily relied on strep tests for virus detection
• Used microscopy to diagnose malaria and various bacterial and parasitic diseases
• Handled sample preparation and analysis with precision to ensure accurate results
• Supported effective diagnosis and treatment for patients
• Worked in a resource-limited hospital setting

Medical Laboratory Technologist, Yastabshiroon Hospital, Khartoum, Sudan

• Analyze blood cell count and conditions
• Test blood and its related groups to determine compatibility
• Conduct chemical analysis of various bodily fluids to discern the presence of abnormal activities
• Used microscopy to diagnose malaria and various bacterial and parasitic diseases
• Identified errors, solved complex laboratory problems, and altered techniques to ensure the elimination of potential discrepancies
• Under minimal supervision:
• Facilitated routine inventory audits
• Maintained laboratory equipment
• Adhered to strict quality control protocols to ensure patient safety and test accuracy
• Procured laboratory consumables